Body fat percentage and bone mineral density(DEXA) 01/Nov/2008 RIKENMPP_022_001 test 0 1 testSt test modification

Bone mineral density (BMD) and body fat are important parameters to assess for bone metabolism deficiencies, lipid metabolism deficiencies, and life-style diseases. BMD and percent body fat (%Fat) are simultaneously measured using a dual-energy x-ray absorptiometry (DEXA) densitometry analyzer (PIXImus2; Luner).

These procedures are applicable to the mouse phenotyping platform at the RIKEN BRC. Testing should be performed by trained staff members who understand the safety and hygiene rules required by RIKEN. Any queries, comments, or suggestions, relating either to this SOP in general or to a specific problem encountered during a procedure, should be addressed to the Blood Tests Unit Leader. Any deviation from this protocol must be reported to the DEXA Tests Unit Leader.

Testing is carried out in accordance with common animal handling procedures. Coveralls (in the rearing facility) or laboratory coats (in the laboratory facility) must be worn with gloves at all times in the work area. If animals are dropped on the floor, treat them in accordance with the safety and hygiene guidelines of the rearing facility at RIKEN. If something unexpected happens during the primary screening, it must be quickly reported to the Blood Tests Unit Leader and recorded. Specific x-ray safety procedures should be followed. Refer to guideline in the resident country for details of x-ray unit applications.

Follow the manual for the PIXImus2 system for quality control.

Dual-energy x-ray absorptiometry (DEXA) densitometry analyzer (PIXImus2;Luner)

Syringe (1 ml) Needle Anesthetic solution

Calculate and record the volume of anesthetic solution required for the intraperitoneal (IP) injection. Administer the anesthetic solution via IP injection. When the mouse becomes unconsciousness, place it into the PIXImus2 analyzer. Follow the manual for the PIXImus2 analyzer to make the measurements.