Genotype Viewer

Analysis Group	Gdf5<Rgsc451>
Analysis Group Description	Phenotypic differences among the three genotypes (Rgsc451/Rgsc451, Rgsc451/+, and +/+) of the B6(D2)-Gdf5<Rgsc451> strain have been analyzed in this analysis group. Test mice were produced by intercross mating between the heterozygote (Rgsc451/+) mice and reared under the JMC standard housing conditions.

Strain	Associated Gene(s)	Housing SOP(s) and SDOP(s)
	B6(D2)-Gdf5<Rgsc451>	Gdf5: growth differentiation factor 5	Mouse husbandry (RIKEN BRC)

Strain	B6(D2)-Gdf5<Rgsc451>	Strain description
Associated Gene(s)	Gdf5: growth differentiation factor 5	This strain has been developed by Hiroshi MASUYA in ENU-mutagenesis project at the RIKEN Genomic Science Center (GSC). A point-mutation of Gdf5 (growth differentiation factor 5) locus has found in the genome of a G1 (DBF1) mutagenized founder mice by the phenotype screen. This strain has been maintained by backcross mating with C57BL/6JJcl strain for 8 generations.
Housing SOP(s) and SDOP(s)	Mouse husbandry (RIKEN BRC)

Strain	Genotype 1	Pipeline(s)
JMC_fundamental_v1
B6(D2)-Gdf5<Rgsc451>	Gdf5^{Rgsc451/Rgsc451}	♀: 10 ♂: 10
Gdf5^Rgsc451/+	♀: 10 ♂: 10
Gdf5^+/+	♀: 10 ♂: 10

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This mutant showed decreased locomotor activity and spent less time in central area of the container in open field test at 7wks. This mutant had short limbs, short digits, and decreased body size at 8wks (modified SHIRPA). Ankylosis of elbows, knees, and digital joints of hind limbs were observed. These phenotypes were more severe in homozygote than heterozygote. A gawky walk was observed in homozygote. Homozygous male could not prolong the latency to fall-down until four trials of the rota-rod test at 12wks. In hematological test at 9wks, the number and ratio of reticulocyte were decreased in hetero female. In clinical biochemical test, the value of three parameters ; CPK, IP, and TG at 11wks and those of two parameters ; T-BIL and TG at 18wks were changed among genotypes. The serum levels of insulin and leptin were decreased in hetero- and homozygote at 18wks. Body fat percentage and body weight was decreased in this mutant at 22wks.

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This mutant showed decreased locomotor activity and spent less time in central area of the container in open field test at 7wks.
This mutant had short limbs, short digits, and decreased body size at 8wks (modified SHIRPA). Ankylosis of elbows, knees, and digital joints of hind limbs were observed. These phenotypes were more severe in homozygote than heterozygote. A gawky walk was observed in homozygote.
Homozygous male could not prolong the latency to fall-down until four trials of the rota-rod test at 12wks.
In hematological test at 9wks, the number and ratio of reticulocyte were decreased in hetero female.
In clinical biochemical test, the value of three parameters ; CPK, IP, and TG at 11wks and those of two parameters ; T-BIL and TG at 18wks were changed among genotypes. The serum levels of insulin and leptin were decreased in hetero- and homozygote at 18wks.
Body fat percentage and body weight was decreased in this mutant at 22wks.